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| Registros recuperados: 11 | |
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| Quadros,V.L.; Mayer,S.V.; Vogel,F.S.F.; Weiblen,R.; Brum,M.C.S.; Arenhart,S.; Flores,E.F.. |
| Calves born persistently infected with non-cytopathic bovine viral diarrhea virus (ncpBVDV) frequently develop a fatal gastroenteric illness called mucosal disease. Both the original virus (ncpBVDV) and an antigenically identical but cytopathic virus (cpBVDV) can be isolated from animals affected by mucosal disease. Cytopathic BVDVs originate from their ncp counterparts by diverse genetic mechanisms, all leading to the expression of the non-structural polypeptide NS3 as a discrete protein. In contrast, ncpBVDVs express only the large precursor polypeptide, NS2-3, which contains the NS3 sequence within its carboxy-terminal half. We report here the investigation of the mechanism leading to NS3 expression in 41 cpBVDV isolates. An RT-PCR strategy was employed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine viral diarrhea virus; CpBVDV; NS3 gene; Cytopathology; RNA processing. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700012 |
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| Mayer,S.V.; de Quadros,V.L.; Vogel,F.S.F.; Winkelmann,E.R.; Arenhart,S.; Weiblen,R.; Flores,E.F.. |
| Bovine herpesvirus type 5 (BHV-5) is a major agent of meningoencephalitis in cattle and establishes latent infections mainly in sensory nerve ganglia. The distribution of latent BHV-5 DNA in the brain of rabbits prior to and after virus reactivation was studied using a nested PCR. Fifteen rabbits inoculated intranasally with BHV-5 were euthanized 60 days post-inoculation (group A, N = 8) or submitted to dexamethasone treatment (2.6 mg kg-1 day-1, im, for 5 days) and euthanized 60 days later (group B, N = 7) for tissue examination. Two groups of BHV-1-infected rabbits (C, N = 3 and D, N = 3) submitted to each treatment were used as controls. Viral DNA of group A rabbits was consistently detected in trigeminal ganglia (8/8), frequently in cerebellum (5/8),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus type 5; BHV-5; BHV-1; Latent infection; Rabbits; Brain. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300004 |
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| Flores,E.F.; Weiblen,R.; Gil,L.H.V.G.; Tobias,F.L.; Lima,M.; Garcez,D.C.; Botton,S.A.. |
| Seqüenciamento e análise filogenética de 17 amostras do vírus da diarréia viral bovina (BVDV) isoladas no Brasil identificaram quatro amostras (23,5%) do genótipo 1a (BVDV-1a), nove amostras (52,9%) do genótipo 1b (BVDV tipo 1b) e quatro amostras (23,5%) do genótipo 2 (BVDV tipo 2). As amostras brasileiras de BVDV tipo 2 apresentaram-se genotipicamente distintas dos BVDV tipo 2 até então identificados na América do Norte e Europa, sugerindo pertencerem a um novo subgenótipo. A caracterização antigênica dessas amostras por neutralização cruzada revelou reatividade sorológica muito reduzida com cepas vacinais do BVDV. O anti-soro produzido contra três cepas vacinais do BVDV apresentou atividade neutralizante muito reduzida contra várias amostras brasileiras... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vírus da diarréia viral bovina; BVDV; Diversidade antigênica; Vacina; Genótipo. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100003 |
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| Anziliero,D.; Weiblen,R.; Kreutz,L.C.; Spilki,F.; Flores,E.F.. |
| The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytokine; MRNA; ORF virus; Immunostimulant; Innate immune response. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200110 |
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| Oldoni,I.; Weiblen,R.; Inkelmann,M.A.; Flores,E.F.. |
| Antigens of a bovine herpesvirus type 5 (BHV-5), isolated from a cow with a neurological infection in Rio Grande do Sul State, Brazil, were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs). Eleven hybridomas secreting mAbs directed at BHV-5 antigens were obtained after two fusions and screening of 356 hypoxanthine-aminopterin-thymidine-resistant clones. The mAbs reacted at dilutions up to 1:500 (hybridoma culture supernatant) and up to >1:10,000 (ascitic fluid) in an indirect fluorescent antibody assay (IFA) and in immunoperoxidase staining of BHV-5-infected cells. Four mAbs (1D12, 2E2, 2G10 and 4E4) showed virus-neutralizing activity against the parental BHV-5 isolate. Five mAbs (1F3, 2A6, 2F9, 2G10 and HB24L) reacted in Western... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus type 5 and type 1; BHV-5; BHV-1; Monoclonal antibodies. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200008 |
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| Kreutz,L.C.; Donis,R.; Gil,L.H.V.; Lima,M.; Hoffman,A.N.; Garcez,D.C.; Flores,E.F.; Weiblen,R.. |
| Three Brazilian isolates of bovine viral diarrhea virus (BVDV), antigenically distinct from the standard North American isolates, were selected to immunize BALB/c mice in order to obtain hybridoma cells secreting anti-BVDV monoclonal antibodies (mAbs). Two hybridoma clones secreting mAbs, reacting specifically with BVDV-infected cells (mAbs 3.1C4 and 6.F11), were selected after five fusions and screening of 1001 hypoxanthine-aminopterin-thymidine-resistant clones. These mAbs reacted in an indirect fluorescent antibody (IFA) assay with all 39 South and North American BVDV field isolates and reference strains available in our laboratory, yet failed to recognize other pestiviruses, namely the hog cholera virus. The mAbs reacted at dilutions up to 1:25,600... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine viral diarrhea virus; Monoclonal antibodies; Antigenic diversity; Diagnostic reagents. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200010 |
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| Traesel,C.K.; Bernardes,L.M.; Spilki,F.R.; Weiblen,R.; Flores,E.F.. |
| Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) share high genetic and antigenic similarities, but exhibit marked differences in tissue tropism and neurovirulence. The amino-terminal region of glycoprotein C (gC), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system. This study investigated the genetic and antigenic differences of the 5′ region of the gC (5′ gC) gene (amino-terminal) of South American BoHV-1 (n=19) and BoHV-5 (n=25) isolates. Sequence alignments of 374 nucleotides (104 amino acids) revealed mean similarity levels of 97.3 and 94.2% among BoHV-1 gC (gC1), respectively, 96.8 and 95.6% among BoHV-5 gC (gC5), and 62 and 53.3% between gC1 and gC5.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GC amino-terminal; BoHV; Genetic diversity; Epitope prediction; Virus neutralization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500470 |
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| Registros recuperados: 11 | |
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